Measurement

Part:BBa_K2406051:Design

Designed by: Jack T. Suitor   Group: iGEM17_Edinburgh_UG   (2017-09-29)


Rox-Term-Rox Measurement construct.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 876
    Illegal AgeI site found at 988
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part required design of specialist PCR primers.


Source

This part was created through round-the-horn PCR of the BBa_J04450 part. Rox-Term-Rox were overhangs on the primers.

References